ABSTRACT
The chromatic values of the broken-fried and single-fried Gardeniae Fructus Praeparatus(GFP) were measured by the color analyzer to analyze the color variation rule, and the contents of 10 main components were determined by ultra-high performance liquid chromatography(UPLC). The multivariate statistical analysis, Pearson correlation analysis, and discriminant analysis were conducted to investigate the color and components of GFP samples. The experimental results revealed that L~*, a~*, b~*, and E~*ab decreased continuously during processing, and the color of samples gradually deepened. The trend and range of chromatic values during broken-frying and single-frying processes were basically identical. Gardenoside, crocin-Ⅰ(C-Ⅰ), and crocin-Ⅱ(C-Ⅱ) showed an obviously downward trend, while the contents of geniposidic acid and 5-hydroxymethylfurfural(5-HMF) increased significantly. Shanzhiside, deacetyl-asperulosidic acid methyl ester, and geniposide(G2) showed a downward trend. Scandoside methyl ester rose first and fell later. Genipin-1-O-gentiobioside(G1) went through a decrease-increase-decrease trend. The change trends of component contents during broken-frying and single-frying processes were generally consistent, but the change range was different. Among all the components, scandoside methyl ester and G1 showed obvious change. Because of different stir-frying time, the change rate of each component content in the process of broken-frying was higher than that in single-frying process. Additionally, geniposidic acid, gardenoside, scandoside methyl ester, C-Ⅰ, C-Ⅱ, and 5-HMF exhibited a higher correlation with apparent color. On the basis of above findings, the discriminant function of two frying processes was established, which could be applied to the discrimination of broken-fried and single-fried samples. This study analyzed the dynamic quality change rule of GFP during broken-frying and single-frying processes based on color-component correlation analysis, and found the two methods showed consistent change trend, yet with slight difference in the quality of samples. This study can provide data support for the processing of GFP.
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Fruit , GardeniaABSTRACT
The chromaticity space parameters of the samples during the processing of Gardeniae Fructus Praeparatus(Jiaozhizi in Chinese herbal name, JZZ) were measured by the visual analyzer to analyze the color variation rule during the processing of JZZ, and the content changes of total reducing sugar, total amino acid and 5-hydroxymethylfurfural(5-HMF) related to Maillard reaction were measured. Pearson correlation analysis and linear regression analysis of the data were carried out by SPSS 24.0 software. The experimental results showed that the objective coloration of the samples in the processing of JZZ was basically consistent with the traditional subjective color judgment; the contents of total reducing sugar and total amino acids showed a decreasing trend during the processing of JZZ, and the content of 5-HMF showed an increasing trend, which was in line with Maillard reaction law. Pearson correlation analysis results showed that there was a significant correlation between the chromaticity space parameters L~*(lightness value), a~*(red green value), b~*(yellow blue value), E~*ab(total color value) and the contents of total reducing sugar, total amino acid and 5-HMF(P<0.01), among which the values of L~*, a~*, b~*, E~*ab were positively correlated with the contents of total reducing sugar and total amino acid, and negatively correlated with the contents of 5-HMF. The results of linear regression analysis also showed that these two were highly correlated. In this study, by establishing the correspondence relationship between the color change of JZZ processing and Maillard reactants, wecan not only provide a basis for the objective digital expression of subjective color of JZZ, but also provide a reference for explaining the processing mechanism of JZZ from a new perspective.
Subject(s)
Amino Acids , Color , Drugs, Chinese Herbal , Fruit , Gardenia , Maillard ReactionABSTRACT
Objective: To study the correlation between the apparent color of the samples and the important variable components of HPLC fingerprint of rice-processed Codonopsis Radix (rpCR) in processing and its quality transfer law, and lay the foundation for revealing the processing principle of rpCR. Methods: This experiment was based on the experience of the process of rpCR and the apparent color change of traditional processed slices, the visual analyzer was used to analyze the change of apparent color of rpCR from different process time. HPLC fingerprint was used to evaluate the dynamic changes of chemical constituents of rpCR in processing. Then, the multivariate statistical method was used to analyze the correlation between the apparent color and the important variable components of the HPLC fingerprint. Results: In the processing of rpCR, the total color value (E*) of sample powder showed a downward trend, the apparent color of the samples changed from light yellowish brown to dark yellow; The proportions of the 10 chemical components varied greatly, corresponding to peaks of 7, 9, 10, 2, 3, 1, 20, 15, 6, and 4 in HPLC fingerprint, among them peak 4 was confirmed to be 5-hydroxymethyl-2-furaldehyde; Components 1, 3 and 4 were highly significantly and negatively relevant with the change of total color value Eab*, and peak 2 was highly significantly and positively correlated with the change of total color value Eab*, while peak 6 and peak 15 were positively relevant with the change of total color value Eab*. Conclusion: Compounds 1, 2, 3, 4, 6, and 15 were the components with obvious changes in process of rpCR, which were significantly correlated with the change of color and could be used as chemical markers for the monitoring processing and quality control of rpCR.
ABSTRACT
RESUMO A água de abastecimento público pode ser um veículo de doenças e agravos à saúde humana, portanto, é necessário um tratamento eficiente e constantes avaliações da sua qualidade. O presente estudo objetivou avaliar a dinâmica populacional de indicadores microbiológicos e parâmetros físico-químicos da qualidade da água em diferentes pontos de uma estação de tratamento de água do tipo convencional, do sistema de distribuição e dos mananciais de abastecimento do município de Jaboticabal, São Paulo, nas estações chuvosa e de seca. Os resultados demonstraram que, apesar do manancial superficial apresentar qualidade microbiológica inferior (comparado aos demais mananciais estudados), após tratamento convencional foi obtida a potabilidade do mesmo. A estação chuvosa foi crítica para amostras coletadas nas etapas logo após adição de cloro, principalmente no sistema de distribuição do manancial subsuperficial. Dentre os pontos avaliados na rede de distribuição, os reservatórios domiciliares apresentaram o maior número de amostras fora do padrão de potabilidade, principalmente na rede abastecida pelo manancial subsuperficial. São necessárias estratégias para a melhoria do processo de tratamento da água do dreno - voltado para a redução da turbidez - principalmente na estação chuvosa; assim como programas de educação em saúde para a população, a fim de melhorar a qualidade da água no ponto de consumo, a partir da limpeza periódica dos reservatórios domiciliares.
ABSTRACT The public-supply water can be a vehicle of disease and harm to human health, therefore, efficient treatment and constant evaluation of its quality is required. The present study aimed to evaluate the population dynamics of microbiological and physico-chemical indicators of water quality parameters at different points of a water treatment plant of the conventional type, in the distribution system and sources of supply in Jaboticabal city, São Paulo state, Brazil, in the rainy and dry seasons. The results showed that although the present fountain surface presents worse microbiological quality (compared to other sources studied) after the conventional treatment, it became potable. The rainy season was critical for samples collected in steps after adding chlorine, especially in the distribution of sub-surface source system. Among the evaluated points in the distribution network, domestic containers had the highest number of samples outside the potability standards, mainly those fueled by the sub-surface source network. Strategies to improve the treatment process of the drain water (turbidity reduction) are needed, especially during the rainy season; as well as health education programs in order to improve water quality at the point of consumption by periodic cleaning of domestic containers.